產(chǎn)品貨號

CP004

反應種屬

Mouse

同種型

Mouse IgG1(switched from rat IgG2a)

實驗應用

in vivo blocking of PD-1/PD-L signaling*

in vitro PD-1 neutralization*

Immunohistochemistry (frozen)*

Immunofluorescence*

Western blot*

Flow cytometry*

*Reported for the original rat IgG2a 29F.1A12 antibody

純度

>95%, Determined by SDS-PAGE

無菌處理

0.2 µm filtration

分子量

150 kDa

保存條件

抗體原液保存在4°C，不能冷凍保存。

推薦抗體稀釋液

InVivoPure pH 7.0 Dilution Buffer（貨號IP0070）

體內(nèi)PD-1/PD-L信號阻斷

(in vivo blocking of PD-1/

PD-L signaling)

1. Wang, W., et al. (2018). 'RIP1 Kinase Drives Macrophage-Mediated Adaptive Immune 

Tolerance in Pancreatic Cancer' Cancer Cell 34(5): 757-774 e757.

2. Gordon, S. R., et al. (2017). 'PD-1 expression by tumour-associated macrophages 

inhibits phagocytosis and tumour immunity' Nature 545(7655): 495-499.

3. Koyama, S., et al. (2016). 'STK11/LKB1 Deficiency Promotes Neutrophil Recruitment 

and Proinflammatory Cytokine Production to Suppress T-cell Activity in the Lung Tumor 

Microenvironment' Cancer Res 76(5): 999-1008.

體外PD-1中和

（in vitro PD-1 neutralization）

1.Park, S. J., et al. (2014). 'Negative role of inducible PD-1 on survival of activated 

dendritic cells' J Leukoc Biol 95(4): 621-629.

流式細胞術

（Flow Cytometry）

1.Good-Jacobson, K. L., et al. (2012). 'CD80 expression on B cells regulates murine 

T follicular helper development, germinal center B cell survival, and plasma cell 

generation' J Immunol 188(9): 4217-4225.