Epicypher新品 | 核酸提取緩沖液

CUTANA™ 細(xì)胞核提取緩沖液是CUT&RUN和CUT&Tag實(shí)驗(yàn)中，用于從培養(yǎng)的細(xì)胞和組織中提取細(xì)胞核的必需試劑。這種緩沖液可與多種真核細(xì)胞和組織兼容。以下是使用該緩沖液成功進(jìn)行細(xì)胞核分離的細(xì)胞和組織類型：

只需將試劑盒中的Pre-Nuclei Extraction Buffer和亞精胺與蛋白酶抑制劑混合即可完成該試劑的制備。CUTANA™ 細(xì)胞核提取緩沖液針對(duì)CUT&RUN和CUT&Tag實(shí)驗(yàn)流程進(jìn)行了優(yōu)化，從而獲得高質(zhì)量的細(xì)胞核，助力您的基因組圖譜分析。

試劑盒組成

保存條件

將Pre-Nuclei Extraction Buffer 4oC儲(chǔ)存, 1 M亞精胺-20oC儲(chǔ)存。

自收到之日起可穩(wěn)定儲(chǔ)存6個(gè)月。

驗(yàn)證數(shù)據(jù)

Figure 1: Nuclei Extraction

Nuclei were extracted from 3 different cell types: K562, NIH3T3, and LNCaP using the CUTANA™ Nuclei Extraction Protocol for CUT&RUN and CUT&Tag. Top 3 panels show cells before extraction. Starting cells are viable (bright white and round). Bottom 3 panels show nuclei extracted using the CUTANA™ Nuclei Extraction Buffer as indicated by positive Trypan Blue staining.

訂購(gòu)詳情

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